Fig. 1.

Antifungal effect of column fractions obtained from T1 system on C. gloeosporioides growth. Hexane-extracted compounds synthesized by co-inoculated B. subtilis, Trichoderma T1 strain, and inactivated C. gloeosporioides mycelium (T1 system) were fractionated by column chromatography. Paper discs (1–7 in E) impregnated with 150 µg/mL of each T1 column fraction (1–35) were placed clockwise, 3 cm apart on a PDA-containing Petri dish with a propagule of C. gloeosporioides (P) in the center. A DMSO-impregnated disc was used as a control (C). The Petri dish was incubated at 4 °C for 24 h and then at 32 ºC for 9 days. Representative images of developed C. gloeosporioides mycelia are shown (G1–G5). This experiment was performed in triplicate