Fig. 2.

Antifungal effect of column fractions from T2 system on C. gloeosporioides growth. Hexane-extracted compounds synthesized by co-inoculated B. subtilis, Trichoderma T2 strain, and inactivated C. gloeosporioides mycelium (T2 system) were fractionated by column chromatography. Paper discs (1–7 in E) impregnated with 150 µg/mL of each T2 column fraction (1–77) were placed clockwise, 3 cm apart on a PDA-containing Petri dish with a propagule of C. gloeosporioides (P) in the center. A DMSO-impregnated disc was used as a control (C). The Petri dish was incubated at 4 °C for 24 h and then at 32 ºC for 9 days. Representative images of C. gloeosporioides mycelia are shown (G1–G11). Discs impregnated with each column fraction were placed clockwise and numbered consecutively in groups of seven fractions according to the scheme (E). This experiment was performed in triplicate