Fig. 3.

Antifungal effect of column fractions from T3 system on C. gloeosporioides growth. Hexane-extracted compounds synthesized by co-inoculated B. subtilis, Trichoderma T3 strain, and inactivated C. gloeosporioides mycelium (T3 system) were fractionated by column chromatography. Paper discs (1–7 in E) impregnated with 150 µg/mL of each column fraction (1–42) were placed clockwise, 3 cm apart on a PDA-containing Petri dish with a propagule of C. gloeosporioides (P) in the center. A DMSO-impregnated disc was used as a control (C). The Petri dish was incubated at 4 °C for 24 h and then at 32 °C for 9 days. Representative images of developed C. gloeosporioides mycelia are shown (G1–G6). Discs impregnated with each column fraction were placed clockwise and numbered consecutively in groups of seven fractions. This experiment was performed in triplicate