Fig. 4.

Antifungal effect of TLC fractions obtained from the T1 system on C. gloeosporioides growth. Hexane-extracted compounds synthesized by co-inoculated B. subtilis, Trichoderma T1 strain, and inactivated C. gloeosporioides mycelium (T1 system) were fractionated with TLC. Paper discs impregnated with 0.5, 1.0, 1.5, and 2.0 μL of the TLC fractions were placed clockwise on a Petri dish (E). A propagule of C. gloeosporioides (P) was placed in the center, 3 cm away from the discs. A DMSO-impregnated disc was used as a control (C). The Petri dish was incubated at 4 °C for 24 h and then at 32 °C for 9 days. Representative images of developed C. gloeosporioides mycelia (1, 4, 5, 6, 7, 8, and 9). Only those images of TLC fractions with a demonstrated effect on pathogen growth are shown. This experiment was performed in triplicate