Fig. 5.

Antifungal effect of TLC fractions obtained T2 system on C. gloeosporioides growth. Hexane-extracted compounds synthesized by co-inoculated B. subtilis, Trichoderma T2 strain, and inactivated C. gloeosporioides mycelium (T2 system) were fractionated with TLC. Paper discs impregnated with 0.5, 1.0, 1.5, and 2.0 μL of the TLC fractions were placed clockwise on a Petri dish (E). A propagule of C. gloeosporioides (P) was placed in the center, 3 cm away from the discs. A DMSO-impregnated disc was used as a control (C). The Petri dish was incubated at 4 °C for 24 h and then at 32 °C for 9 days. Representative images of developed C. gloeosporioides mycelia (3, 4, 6, 10, 11, 14, and 18). Only those images of TLC fractions with a demonstrated effect on pathogen growth are shown (3, 4, 6, 10, 11, 14, and 18). This experiment was performed in triplicate