Fig. 6.

Antifungal effect of TLC fractions from T3 system on C. gloeosporioides growth. Hexane-extracted compounds synthesized by co-inoculated B. subtilis, Trichoderma T3 strain, and inactivated C. gloeosporioides mycelium (T3 system) were fractionated with TLC. Paper discs impregnated with 0.5, 1.0, 1.5, and 2.0 μL of the TLC fractions were placed clockwise on a Petri dish (E). A propagule of C. gloeosporioides (P) was placed in the center, 3 cm away from the discs. The Petri dish was incubated at 4 °C for 24 h and then at 32 °C for 9 days. A DMSO-impregnated disc was used as a control (C). Representative images of developed C. gloeosporioides mycelia (1, 2, 3, 6, 8, and 10). Only those images of TLC fractions with a demonstrated effect on pathogen growth are shown (1, 2, 3, 6, 8, and 10). This experiment was performed in triplicate