Fig. 8.

Effect of butyl acetate on germination of C. gloeosporioides spores. a Representative micrographs of mycelium from spores germinated in PDB medium. Spores prepared from C. gloeosporioides were grown in PDB supplemented with ethanol (0) and indicated concentrations of butyl acetate for 10 days at room temperature in the dark. Mycelial samples were stained with a drop of brilliant blue and then visualized under a microscope using the 40 × objective. Samples were taken 1 day or 2 days after inoculation (dai). b Representative images of C. gloeosporioides grown in PDB alone for 10 days. Spores were prepared from C. gloeosporioides grown on PDA (control), PDA supplemented with ethanol, or PDA supplemented with 100 µM of butyl acetate for 10 days at room temperature in the dark. c Representative micrographs of the mycelium from b. Mycelial samples were stained with a drop of brilliant blue and visualized under a microscope with 10 × and 40 × objectives