Fig. 2.
In vitro and in vivo infectivity of SHIV162P3-infected splenocytes. (a) Titration of splenocytes in coculture with PBMCs. Viral production was detected by RT-qPCR 7 days post-coculture. (b) Titration of splenocytes in coculture with TZM-bl cells. Infection was recorded as Relative Light Unit (RLU) at 48 h post-coculture after subtraction of the RLU of the control wells (TZM-bl only). Each bar represents the mean and standard error of the mean (SEM) of three or four independent experiments performed in triplicate. Each color represents a different animal. * p = 0.0385, ** p = 0.0039, non-parametric Mann Whitney test. (c) Follow-up of blood viral RNA load of two macaques (CE470 in violet and CE468 in pink) intravaginally exposed to 107 spleen cells from #AX450. Arrows represent weekly inoculations of splenocytes, performed for three weeks for CE470 and six weeks for CE468.