Fig. 3.
Neutralization of SHIV162P3 cell-cell transmission by 2F5, 4E10, and 2G12 bNAbs used singly or in double or triple combinations. (a) TZM-bl neutralization assay. Results after 48 h of splenocyte/TZM-bl coculture are shown. (b) PBMC-based neutralization assay. Infection was analyzed by SIV p27 Ag ELISA seven days post-coculture. The mean and SEM of three to four independent experiments is shown. Dotted lines indicate 0, 50, and 90% inhibition viral transmission. Antibodies targeting the N-glycan of the gp120 (2G12) and the MPER (2F5 and 4E10) of the gp41 were used.