Fig. 5.
bNAb-mediated inhibition of semen leukocyte SHIV162P3 transmission to target cells. (a) Follow-up of blood viral RNA load (BVL, lines) and semen viral load (SVL, histogram) on the day of euthanasia of four macaques infected with SHIV162P3. Each color represents a different macaque. (b) TZM-bl infection by either 40,000 CD45+ semen cells or 40,000 spleen cells in presence and absence of 132 µg/ml of 2F5+2G12+4E10 Ab pool (targeting the N-glycan of the gp120 and the MPER of the gp41). Mean and SEM of one representative experiment performed in duplicate is shown. (c) The anti-V3 10–1074 Ab mediated inhibition of transmission from CD45+ semen cells to PBMCs. Infection was analyzed by RT-qPCR seven days post-coculture. Each condition was assessed in duplicate. The mean and SEM of three independent experiments, with semen cells purified from three different donors is shown.