Table 3.

The prothrombin time (PT) and the activated partial thromboplastin time (APTT) of human plasma in presence of various concentrations of crude extract of F. carica latex proteases and purified FPIII.

PT at different concentrations				APTT at different concentrations
Crude extract of F. carica proteases		FPIII		Crude extract of F. carica proteases		FPIII
μg proteins	PT Sec.	μg proteins (0.4 μM)	PT Sec.	μg proteins	APTT Sec.	μg proteins (0.4 μM)	APTT Sec.
13	15 ± 0.6	5.6	>300	13	14 ± 0.5	5.6	>300
26	18 ± 0.6			26	26 ± 1.0
39	25 ± 1.0			39	38 ± 1.5
52	35 ± 2.1			52	49 ± 2.0
65	59 ± 1.5			65	63 ± 2.0
78	82 ± 2.5			78	97 ± 3.0
91	128 ± 3.0			91	175 ± 1.5
104	200 ± 4.0			104	219 ± 3.0
117	263 ± 3.2			117	270 ± 2.0
control		30		control		47

The different concentrations of crude extract of F. carica proteases and FPIII were pre-incubated with 10 mM BaCl₂. The control is the clotting time of the human plasma contained 10 mM BaCl₂ in absence of anticoagulant. n = 3. The results are presented as the mean ± SD.