Fig. 3.

Culturing primary human fibroblasts on top of (a, d) and within 3D fibrin matrices (b, c, e). a) The nuclei of cells attached to fibrin matrices at 3 h. b) Fibroblasts in 3D fibrin matrices at 2 h and 48 h. c) Live and dead cells at 48 h. d) Cells attached to the fibrin matrices were quantified with alamar blue assay. e) Cells in 100 μl fibrin matrices at 48 h were quantified with alamar blue assay. Cell numbers are related to the initial seeded cells. **: p < 0.01.