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. 2020 Jun 20;23(7):101290. doi: 10.1016/j.isci.2020.101290

Figure 2.

Figure 2

Targeting Regions of Interest by Semithick Sectioning

(A) Cartoon of the information content of BSD surface imaging and the potential FIB-SEM analysis of sections of different thickness.

(B and C) (B) Transmitted light and (C) BSD images of semithin 0.5-μm and semithick 2-, 5-, and 10-μm sections of mouse brain tissue. cc, corpus callosum; ct, cortex; CA1, hippocampus CA1. Arrowheads indicate cracks, mainly in heavily stained tissue (corpus callosum). Scale bars 100 μm (top), 10 μm (bottom).

(D) Consecutive (upper, lower row) semithick (2, 5, 10 μm) sections allow tracing cell bodies and blood vessels across sections. Somata and blood vessels that can be followed from one to the next section are colored blue; others are shown in orange. Black scale bar 10 μm; white scale bars 2 (left), 5 (middle), and 10 μm (right). See Figure S1 for similarity of opposing surfaces of consecutive sections.