Figure 6.

AT-GAA Improved AMPK-TSC2 Signaling in Muscle from KO Mice

Muscle biopsies were collected from age and sex-matched WT, untreated (KO), and AT-GAA-treated KO (KO-ERT) mice. (A) Western blot analysis of muscle lysates from WT, untreated KO (KO), and treated KO (KO-ERT) mice with the indicated antibodies (n = 4 for each group for LKB1; n = 6 for each group for p-AMPKα T¹⁷²). (B and C) Western blot analysis of muscle lysates from WT, untreated KO (KO), and treated KO (KO-ERT) mice with the indicated antibodies (n = 4 for each group). GAPDH was used as loading control. Each data point represents an individual mouse. (D) A diagram showing the position of the analyzed proteins. Statistical significance was determined by one-way ANOVA. Graphs represent mean ± SD. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001.