Table 1.
Primer sequences for real-teime PCR.
| Gene name |
Gene symbol |
Forward Primers (5′ to 3′) |
Reverse Primers (5′ to 3′) |
|---|---|---|---|
| Liver | |||
| Glucose uptake | |||
| Solute carrier family 2 (facilitated glucose transporter), member 1 (Slc2a1) | Slc2a1 | GACCCTGCATCTGATTGGTCTG | CCACAATGAACCATGGAATAGGA |
| Solute carrier family 2 (facilitated glucose transporter), member 2 (Slc2a2) | Slc2a2 | TTGGTGCCATCAACATGATCTTC | AGATGGCCGTCATGCTCACATA |
| Glycolysis | |||
| Glucokinase | Gck | AGTATGACCGGATGGTGGATGAA | CCAGCTTAAGCAGCACAAGTCGTA |
| Phosphofructokinase | Pfkl | CCACCTGGAGGCCATTGATGA | GGGATGACGCACATGACGA |
| Pyruvate kinase | Pklr | ATCTGGGCAGATGATGTGGA | ATAGGGTGTAACTGGGTCAGAATGG |
| Glycogen metabolism | |||
| Glycogen synthase 2 | Gys2 | CATGAATGGCAGGCTGGAAC | GCTCCATGCAGTAGCGGTGA |
| Glycogen phosphorylase | Pygl | GATCCGCACACAGCAGCACTA | CTTCGTCGCAGGCATTCTGTAA |
| Gluconeogenesis | |||
| Glucose-6-phosphatase | G6pc | TTAGAGGCAAAGGAGCCCAAG | GGGTGGAAACACAGGCATCA |
| Phosphoenolpyruvate carboxykinase 1 | Pck1 | CAGCCAATGTCCCATTATTGACC | TGCCAGCTGAGAGCTTCGTAGA |
| Soleus muscle | |||
| Glucose uptake | |||
| Solute carrier family 4 (facilitated glucose transporter), member 4 (Slc2a4) | Slc2a4 | CTCCAACTGGACCTGTAACTTCATC | GCCTCTGGTTTCAGGCACTC |
| Glycolysis | |||
| Hexokinase 1 | Hk1 | ATTGTCGCCGTGGTGAATGA | TAGCAAGCATTGGTGCCTGTG |
| Hexokinase 2 | Hk2 | TCGATGGCTCCGTCTACAAGAA | ACATCACAGTCGGGCACCAG |
| Phosphofructokinase | Pfkm | GGGCTGACACAGCACTGAACA | GGCCAGATAGCCACAGTAACCAC |
| Pyruvate kinase | Pkm | TGTTTAGCAGCAGCTTTGATAGTTC | GCGTGTCACAGCAATGATAGGAG |
| Glycogen metabolism | |||
| Glycogen synthase 1 | Gys1 | TCAGAGCAAAGCACGAATCCAG | AACTCATAGCGTCCAGCGATAAAGA |
| Glycogen phosphorylase | Pygm | TCCGCACACAGCAGCATTACTAC | TCCAAGGCCAGGTTCACCA |
| Gluconeogenesis | |||
| Phosphoenolpyruvate carboxykinase 1 | Pck1 | CAGCCAATGTCCCATTATTGACC | TGCCAGCTGAGAGCTTCGTAGA |