Table 2.
Purification procedure of the recombinant proteins.
| Protein | Purification steps | Total activity (U) | Total proteins (mg) | Specific activity (U/mg) | Purification (fold) | Recovery yield (%) |
|---|---|---|---|---|---|---|
| LmChiA | Cell free supernatant Ni-NTA chromatography |
1209.6 856.8 |
155.1 7.1 |
7.8 120.2 |
1.0 15.4 |
100.0 70.8 |
| LmChiB | Cell free supernatant Ni-NTA chromatography |
9182.2 3458.6 |
102.2 15.9 |
89.8 217.8 |
1.0 2.4 |
100.0 37.7 |
Assays were performed at 37 °C in sodium phosphate buffer pH 7.4 using 5% colloidal chitin as substrate.