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. 2020 Jul 1;6(7):e04252. doi: 10.1016/j.heliyon.2020.e04252

Table 3.

Specific activity against colloidal chitin and artificial substrates of LmChiA and LmChiB.

Substrate Specific activity (U/μmol)
LmChiA LmChiB
Colloidal chitin∗ 4.85 ± 0.57 17.53 ± 0.58
4-nitrophenyl-N-acetyl-β-D-glucosamine [4NP-(GlcNAc)]∗∗ 0 0
4-nitrophenyl N,N′-diacetyl-β-D-chitobioside [4NP-(GlcNAc)2]∗∗ 20.02 ± 0.86 20.64 ± 1.13
4-nitrophenyl N,N′,N″-triacetyl-β-D-chitotriose [4NP-(GlcNAc)3]∗∗ 22.75 ± 0.53 12.10 ± 0.44
∗

For assays using colloidal chitin substrate (5% final concentration), one unit was defined as the amount of enzyme which released 1 μmol of GlcNAc/mg/min under assay condition.

∗∗

For assays using artificial substrates (0.3 mM final concentration), one unit was defined as the amount of enzyme which released 1 μmol of 4-nitrophenol/mg/min under assay condition.