Table 5.

Specific activity of mutant and wild-type chitinase measured on colloidal chitin substrate.

Enzyme	Specific activity (U/μmol)	Relative specific activity (%)
Wild-type ChiA	2.01 ± 0.08	100
ChiA-D159A	0.66 ± 0.13	32.84
ChiA-D161A	0.75 ± 0.12	37.31
ChiA-E163A	0.62 ± 0.19	30.85
Wild type ChiB	11.61 ± 0.21	100
ChiB-D168A	1.51 ± 0.14	13.01
ChiB-D170A	2.20 ± 0.16	18.95
ChiB-E172A	1.56 ± 0.22	13.44