Fig. 7.

Activity of 237-derived CAR T cells against various mouse and human cancer cell lines. (A) Mock, 237-CAR, WE-CAR, TNGK-CAR, LGQ-CAR, or 5E5-CAR transduced T cells were cocultured with Tn antigen expressing murine target cells (Ag104A or ID8 Cosmc KO) or with control cell lines (ACosmc, ID8, or 58^−/−), for 24 h at 37 °C, 5% CO₂ at various target-to-effector cell ratios. IFN-γ released in the supernatants under each coculture condition was measured by ELISA. Activation of transduced cells was measured in duplicate in two independent experiments. (B) Mock, 237-CAR, WE-CAR, TNGK-CAR, LGQ-CAR, or 5E5-CAR transduced T cells were cocultured with Tn antigen expressing human target cells (Jurkat, Jurkat-MUC1 KO, SKOV3-Cosmc KO, or SKOV3-Cosmc KO-MUC1 KO) or with control cell lines (Jurkat-Cosmc⁺ or SKOV3), for 24 h at 37 °C, 5% CO₂ at various target-to-effector ratios. IFN-γ released in the supernatants under each coculture condition was measured by ELISA. Activation of transduced cells was measured in duplicate in two independent experiments. Error bars indicate SEM. Significance of difference in activity of each CAR compared to control (mock) was determined by two-way ANOVA followed by Dunnett’s test using GraphPad Prism 8.0. ns refers to P ≥ 0.05; * refers to P = 0.01 to 0.05, *** refers to P = 0.0001 to 0.001, and **** refers to P < 0.0001.