Fig. 2.
Identification of an Aspergillus lipid species, whose synthesis requires ErdS. (A) Deletions of erdS from Afm and from Aor were performed by homologous recombination (see SI Appendix for details). The genotypes of the strains are indicated. For Afm, the ΔerdS strain shown corresponds to the strain after excision of the deletion cassette, whereas, for the complemented ΔerdS::PX-erdS strain, the selection marker is still present. For Aor, the ORF of erdS was replaced by a pyrG-containing module and subsequently excised by selection on 5-FOA medium. Complementation was operated by ectopic expression of erdS-ΔDUF, erdS-ΔAspRS, or erdS. (B and C) Total lipids extracted from the different strains described in A were analyzed by TLC and stained with a sulfuric acid/MnCl2 solution. TLC plates were observed either under white light or under UV light. Cultures were done in glucose or xylose containing media; * indicates the LX. (D) Quantification of the TLCs shown in B and C. LX signal (number of pixels) was normalized to that of PE (phosphatidylethanolamine) (LX/PE ratio). All TLCs are representative of at least two independent experiments (n = 2).