Fig. 5.
Inhibiting macrophage glycolysis reduces renal IL-1β and neutrophil recruitment in vivo. (A) Experiment design of the kidney-IgG IC model. (B) qPCR of Il1b, Tnf, Il6, and Ptgs2 mRNA in whole kidney tissue of mice treated with Ova, Ova-IC, or Ova-IC + 2DG. Data are normalized to Ova controls and Hprt. (C) MNP1 and MNP2 gating and quantification in kidneys from mice treated as in A (n = 6 per group). Means ± SEM indicated. (D and E) Intracellular pro–IL-1β staining (D) and quantification (E) by flow cytometry for kidney MNP1 and MNP2 populations from mice treated as in A (n = 6 per group). Medians are indicated. (F) Quantification of CD11b+ Ly6C/Ghi neutrophils in mouse kidneys following Ova or Ova-IC injection with or without the pretreatment of 2DG (n = 6 per group). Medians are indicated. Data are representative of three independent experiments. P values were calculated using the nonparametric Mann–Whitney U test (B, E, and F; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001).