Fig. 5.

Synaptic integration analysis of transplanted hESC-derived DA neurons. Double staining for GFP (green) and mCherry (red) shows the transplanted cells after rabies injection to assess the host integration of the grafted cells (A and B). GFP+/mCherry+ cells (arrowheads in D) show transplanted cells whereas GFP−/mCherry+ cells (arrows in D) show host neurons making synaptic connections with grafted cells. GFP−/mCherry+ were mapped and their number and location are graphically depicted in C where the darker the color, the greater the number of host-connecting neurons in that region. High-magnification images of individual mCherry+ host neurons from the areas of the brain most highly synaptically connected with the graft are shown in E–G. A connectivity ratio of the number of host connections relative to transplanted cells is depicted in H. Ctx, cortex; Hyptha, hypothalamus; mo, motor cortex; PFC, pre-frontal cortex; si, somatosensory cortex. (Scale bars, 100 μm [A and B] and 50 μm [D–G].)