Fig. 1.

Auxin signaling in wound-adjacent cells. (A and B) Wound-adjacent cells exhibit increased auxin signaling compared with neighboring cells. (A) DII-Venus (green) expression (of R2D2-negative auxin marker line) in cells next/adjacent to ablated cells (marked with asterisk) at 1, 3, 11, and 17 h after cell ablation. A lower DII-Venus signal indicates higher auxin signaling. Cell walls and dead cells are stained with PI (pink). (B) Schematic representation of wound-adjacent cells at different stages of regeneration. Ablated cell is in pink with an asterisk. (C) Quantification of DII-Venus fluorescence signal over time from wound-adjacent cells before cell division (red) or after cell division (wound-adjacent in green; non–wound-adjacent in blue). Data are represented as DII-Venus fluorescence in observed cells relative to neighboring, nonadjacent cells. Time “0 h” represents the time of restorative cell division. Thick lines represent the smoothed mean, and the lighter background represents smoothed SE from n = 18 cells each. Statistical significance was computed using a one-sample Wilcoxon test.