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Detection of hepatic LDs fraction under light and fluorescent microscopy. A, micrograph of LDs fraction from liver of control animal under normal light at ×40 magnification. B and C, micrographs of LDs after staining by Nile Blue. Stained LDs were examined under a fluorescent microscope (Nikon Ti-U microscope supplied with an Olympus FE-4000 camera) using green and red fluorescence filters (excitation, 515-560 nm; emission, > 590 nm) at magnification of ×100. Bars represent 10 μm.
