Fig. 4.

AFB₁ modifies the lipid profile of hepatic LDs and the expression of key genes in the oxylipin biosynthesis pathway. A, TLC-analysis of neutral lipids of hepatic LD isolated from control, low- (L)- and high- (H)-dosed animals. Abbreviations: MAG, monoacylglycerol; DAG, diacylglycerol; FFA, free fatty acids; TAG, triacylglycerol; SE; sterol ester. B, relative quantification of transcripts for key genes of the oxylipin biosynthesis pathway. C, enzymatic activity of the LD-associated oxygenases measured by differential absorption at 310 nm. For each set, two independent measurements were taken for three individual animals. Uppercase letters indicate significant differences in the genes expression or enzymatic activity between control and AFB₁-dosed animals (^bP < 0.05).