Figure 3.

dPTPMT1 Depletion Results in Drs Expression in the Trachea

(A and B) (A) qPCR analysis of Drs expression using whole-fly lysates. n = 3–6 replicates of 15–20 0- to 2-day-old adult males (A) or third instars (B). Data are represented as mean ± SEM and ∗∗p < .01, ∗∗∗p < 0.001, by one-tailed Student's t test.

(C) Drs-GFP expression in dPTPMT1 RNAi adult females, 0–2 days old. Arrow points to signal in the leg trachea. Females are heterozygous for y mutation (y/y⁺). Scale bars, 250 μm.

(D) Percentage of adult females with Drs-GFP in the trachea. Females are heterozygous for y mutation (y/y⁺). n = 3 replicates, 17–57 for each repeat, 0–2 days old. Data are represented as mean ± SEM and ∗∗∗p < 0.001 by chi-square test.

(E) Drs-GFP expression in dPTPMT1 RNAi third instar males. Arrow points to Drs-GFP expression in the dorsal trunk of the trachea and posterior spiracles. Dashed box shows a higher magnification image of the affected area. Scale bars, 250 μm.

(F) Percentage of third instar males with Drs-GFP expression in the trachea. n = 4–7 replicates, 12–53 for each repeat.

Data are represented as mean ± SEM and ∗∗∗p < 0.001 by chi-square test. See also Figure S3.