Box 1.
Procedure for obtaining prolactin concentrations from figures.
| 1. The PDF of the figure in the article was copied from Adobe PDF zooming by 600%. To determine the prolactin concentration of a particular value (yn), three positions (in mm) were determined using rules in Microsoft Publisher (zoom view set at 600%): |
| ymax: the maximum concentration of the prolactin scale. We annotated the y position of the Publisher y axis rule (in mm). |
| y0: the minimal concentration of the prolactin scale (usually zero). We annotated the y position of the Publisher y axis rule (in mm). |
| yn: the concentration of the prolactin value to be extracted (unknown). We annotated the y position of the Publisher y axis rule (in mm). |
| 2. Calculation of d1 and d2. |
| d1= y0-ymax (in mm). |
| d2= y0-yn (in mm). |
| 3. We aimed to calculate a ratio that would reflect the increase in prolactin concentrations for each mm of y axis rule increase. |
| Ratio PRL/d1= Range of prolactin concentrations (from y0 to ymax)/d1 |
| 4. Calculation of prolactin concentration with the following formula: |
| PRL (yn)= d2 x Ratio PRL/d1 |
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