Figure 1.

Overview of Experimental Evolution of Resistance in Ewing’s Sarcoma Cell Lines

As cells recovered from each exposure, cells were tested for their sensitivity to a panel of drugs and samples were frozen for potential use in RNA sequencing. The drug dosage was only increased once throughout the experiment, at the fifth exposure to the VDC combination, described in Transparent Methods. Although each cell line began with five experimental and three control evolutionary replicates, the A673 cell line lost one experimental replicate owing to contamination.