Figure 3.

Point-Range Plots Demonstrating EC50 Changes in A673 Experimental and Control Replicates Over Time

Bottom: Point-range plots representing the changes in drug response to a panel of nine drugs. Experimental time points (x axis) represent which step in the drug cycle the replicates have just recovered from. Points on the plot represent the average EC50 for the group, either experimental or control. Lines represent the range for the entire group. The EC50 of olaparib for Replicate 5 after the fifth exposure to VDC is indeterminate owing to a poorly fit dose-response model and has been removed from this drug's VDC5 time point experimental group EC50 average and range calculations. This value has not been censored in Figures S1 and S2. The y axis of all the point-range plots has μM units, except Cyclo, where the unit is percent of chemically activated 4-hydroxycyclophosphamide solution by volume. Top: Two plots demonstrating a more detailed view of the dose-response data represented at the EC3 and VDC5 time points in the Doxo point-range chart. Cellular activity is measured by enzymatic conversion of alamarBlue, normalized to background florescence. Comparing these two plots shows the clear divergence in drug response between experimental and control evolutionary replicates as the treatment regimen continued.