Fig. 6.

Influence of A1120 concentration and soluble hRBP4 on the ON-switch CAR function. Reporter Jurkat cells were electroporated with mRNA encoding both chains I and II of the ON-switch CAR and cocultured with NALM6 target cells (E:T = 1:2) for 20 h. Target cells were blocked with 10% human serum and 10% human IgG for 15 min at 4 °C before effector cells were added. Expression of the fluorescent reporter proteins was analyzed by flow cytometry. In A different concentrations of A1120 were administered to the cocultures, and EC₅₀ values were calculated by fitting the data with a nonlinear regression model with a variable slope using GraphPad. In B the cells were incubated either without compound or with 5 µM A1120 and with or without 1 µM soluble hRBP4. Data shown in A and B are averages ± SDs of three independent experiments.