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. 2020 Jun 3;40:101033. doi: 10.1016/j.molmet.2020.101033

Figure 5.

Figure 5

Insulin downregulates ANGPTL4 in vivo and in vitro. A) Plasma insulin concentration after 2 h (Fed) and 26 h (Fasted) of fasting. Each line represents one individual. Individuals are depicted in the same color in all figures. Bars represent group means (N = 23). B) Adipose tissue mRNA levels of ANGPTL4 and ANGPTL8 in insulin-sensitive (blue lines) and insulin-resistant (orange lines) subjects before and after a hyperinsulinemic clamp. Data were extracted from GSE26637. C) Western blot for ANGPTL4 and GAPDH in primary human subcutaneous adipocytes treated with cycloheximide for different durations. D) Quantification of the ANGPTL4 levels relative to GAPDH. E) ANGPTL4 mRNA in primary human visceral and subcutaneous adipocytes treated with 500 nM insulin for 24 h. F) Western blot for ANGPTL4 and HSP90 in primary human visceral adipocytes treated with insulin. HSP90 was blotted as a loading control. Statistical differences for in vitro experiments were assessed using the unpaired Student's t-test. ∗p < 0.05, relative to control treatment.