Figure 6.

Corticosteroids and fatty acids upregulate ANGPTL4 in primary human adipocytes. (A) Plasma cortisol concentration after 2 h (Fed) and 26 h (Fasted) of fasting. Each line represents one individual. Individuals are depicted in the same color in all figures. Bars represent group means (N = 23). ANGPTL4 mRNA (B) and ANGPTL4 protein levels (C) in primary human visceral adipocytes treated with cortisol (1 μM), dexamethasone (1 μM), or a mixture of oleate and palmitate (2:1, 300 μM total) for 24 h. HSP90 was blotted as a loading control. D) LPL and ANGPTL8 mRNA in primary human subcutaneous adipocytes treated with insulin (500 nM), cortisol (1 μM), or dexamethasone (1 μM) for 24 h. Statistical differences for in vitro experiments were assessed using the unpaired Student's t-test. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, relative to control treatment.