Fig. 5.

Validation of MitoSV-seq in colon cancer cell lines. (a) Single living cell isolation from SW480 and SW620 cell lines. (b) Evaluation of MitoSV-seq in bulk cells vs single cells in SW480 and SW620 cell lines. B: bulk; SC: single cell. (c) Distribution of variations in bulk and single SW620 cells with MitoSV-seq. Venn diagram represents total number of detected variations in each condition and their overlap. Circos plots of human mtDNA genome displaying heavy and light origins of replication (OH and LO, respectively), major arc, and D-loop (outer blue circle). mtDNA genes are displayed in the middle circle (grey lines). Also shown are SVs as arches with deletions (blue), tandem duplications (red), inversions (green, and insertions (orange). Thickness of the arches corresponds to SV heteroplasmy. SNVs are marked with short red lines (_) according to their positions in mouse mtDNA. Intensity of red lines corresponds to SNV heteroplasmy. (d) Identification of SVs and SNVs on treatment of SW480 and SW620 cell lines after mild oxidative stress induction_. T: treated. (e) Comparison of conventional technique with MitoSV-seq in bulk sample. C, conventional technique; N, new (MitoSV-seq).