Figure 1.

P. aeruginosa molecules affect T. cruzi Y strain infection and multiplication. Vero cells (2 × 10⁵/well) were infected with TcY at a ratio of 5 trypanosoma per cell for 24 h at 37°C in 5% CO₂ and 80% humidity. Infections took place in the presence of pure RPMI, AmB (250 ng/mL) or 1:2 dilutions of planktonic PA14 wild-type cell-free supernatants (1:2 to 1:64). Twenty-four hours after infection, cells were washed and fresh medium without PA14 supernatants or TcY was added. (A) Infection rates and (B) multiplication were determined 48 h after infection. (C) Cell viability was determined by XTT assay. Statistics: Welch t test; comparison, RPMI vs all other bars. Asterisks represent significant decreases, squares represent significant increases. One, two or three asterisks or squares: p≤0.05, p≤0.01 or p≤0.001, respectively.