Figure 3.

Pyochelin affects T. cruzi Y strain infection and multiplication. Vero cells (2 × 10⁵/well) were infected with TcY at a ratio of 5 trypanosoma per cell for 24 h at 37°C in 5% CO₂ and 80% humidity. Infections took place in the presence of pure RPMI, BNZ (11.5 or 23 μM), pyochelin (PYOC, 10 μM), pyoverdine (PYOV, 10 μM), PQS (10 μM), C4-HSL (10 μM) or 3-oxo-C12-HSL (10 μM). Twenty-four hours after infection, cells were washed and fresh medium without drugs or TcY was added. (A) Infection rates and (B) multiplication were determined 48 h after infection. (C) Cell viability was determined by XTT assay. Statistics: Welch t test. Comparisons: RPMI vs all other bars. Asterisks represent significant decreases, squares represent significant increases. One, two or three asterisks or squares: p≤0.05, p≤0.01 or p≤0.001, respectively. ns: not significant.