Table 1.
PA14 mutants with significant loss of anti–T. cruzi activity
| No. | Mutant | Mutation result | Reference |
|---|---|---|---|
| 1 | pvdD-/pchE- | Pyoverdine–pyochelin double siderophore mutant | 10 |
| 4 | pqsA- | Gene for anthranilate-CoA ligase lost; loss of extracellular quinolones, HAQ biosynthesis, including HHQ, PQS and DHQ, thus absent or decreased activity of MvfR | 34 |
| 7 | lasR- | Lacks several QS-regulated factors, including proteases, 3-oxo-C12-HSL; delayed activation of RhlR QS pathway, less pyoverdine | 17 |
| 8 | rsmA- | Global post-transcriptional regulator mutant | 35 |
| 12 | HSI 1/2 | Double mutant defective in two of three type VI secretion systems | 36 |
| 15 | phzC1-/phzC2- | Double phenazine mutant (completely abrogated), no pyocyanin | –a |
| 16 | pchE- | Loss of pyochelin (siderophore) mutant | 18 |
| 21 | pqsA-pqsH- polar | ED2, pqsA::TnPhoA, pqsH::Gm, the mutation in pqsA is polar, kan and gm resistant | –a |
| 22 | chiC- | Loss of chitinase | 18 |
| 23 | lecA- | Loss of galactose-specific lectin A | 18 |
| 24 | hcnA- | No hydrogen cyanide production | 18 |
| 26 | lasI- | No 3-oxo-C12-HSL production | 14 |
aUnpublished lab strain (available from E. Deziel).