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. 2020 Jul 3;18:82. doi: 10.1186/s12915-020-00816-1

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Genetic rescue of Dcr-2 expression recovers TBPH mutant pathological phenotypes. a Number of peristaltic waves of Ctrl (w¹¹¹⁸), Δtb-driver>GFP (tbph^Δ23/tbph^Δ23;driver-GAL4/UAS-GFP), and Δtb-driver>Dcr-2 (UAS-Dcr-2/+;tbph^Δ23/tbph^Δ23;driver-GAL4/+). elav-GAL4, D42-GAL4, and repo-GAL4 were used as reported on the figure. n = 20. b Confocal images of third instar NMJ terminals in muscle 6/7 second segment stained with anti-HRP (in green) in Ctrl, Δtb-driver>GFP, and Δtb-driver>Dcr-2, using elav-GAL4. c Quantification of branch number. n = 15. d Confocal images of third instar NMJ terminals in muscle 6/7 second segment stained with anti-HRP (in green) and anti-GluRIIA (in magenta) in Ctrl, Δtb-driver>GFP, and Δtb-driver>Dcr-2, using elav-GAL4. e Quantification of GluRIIA intensity. n > 200 boutons. f Real-time PCR of gypsy transcript levels normalized on Rpl11 (housekeeping) in Ctrl, Δtb-driver>GFP, and Δtb-driver>Dcr-2, using elav-GAL4. n = 2, error bars SEM. g Confocal images of third instar NMJ terminals in muscle 6/7 second segment stained with anti-HRP (in magenta) for presynaptic membrane and anti-GFP (in green) for peripheral glia in Ctrl (+/+;repo-GAL4,UAS-GFP/+), Δtb-GFP (tbph^Δ23/tbph^Δ23;repo-GAL4,UAS-GFP/+), and Δtb-Dcr-2 (UAS-Dcr-2/+;tbph^Δ23/tbph^Δ23;repo-GAL4,UAS-GFP/+). h Quantification of glial area. n = 10 larvae. j Quantification of branch number. n = 10. k Number of peristaltic waves of GFPi (Dcr-2/+; tbph^Δ23,elav-GAL4/+;UAS-GFP-IR/+) and TBi (Dcr-2/+; tbph^Δ23,elav-GAL4/+;UAS-TBPH-IR/+) fed with 10 μM enoxacin (D) compared to vehicle only (V). n = 20. l Confocal images of third instar NMJ terminals in muscle 6/7 second segment stained with anti-HRP (in green) and anti-GluRIIA (in magenta) in GFPi and TBi. m Quantification of GluRIIA intensity. n > 200 boutons. *p < 0.05, **p < 0.01, ***p < 0.001 calculated by one-way ANOVA, error bars SEM. Scale bar 10 μm (b, g) and 5 μm (d, l)