Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 May 2;5(1):bpaa009. doi: 10.1093/biomethods/bpaa009

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Figure 2: — Optimization of Chelex^® 100 resin-based extraction. Replicates of five were conducted for each condition in a single experiment. (A) Second overnight lysis does not increase the total gDNA yield. The eluant for each lysis was collected separately. The total double lysis yield was calculated by adding together the DNA yield from both elutions. The total DNA from each elution and the total is represented normalized to the control ± standard error. (B) Second 95°C heat precipitation significantly increases the total gDNA yield. The eluant for each heat precipitation was collected separately. The total double heat precipitation yield was calculated by adding together the DNA yield from both elutions. The DNA from each elution and the total is represented normalized to the control ± standard error. (C) Absolute efficiency of control versus optimized protocols. For the optimized protocol, each eluant from the heat precipitation was collected separately. The total optimized double precipitation was calculated by adding together the DNA yield from both elutions. Data from each elution and the total is represented as mean DNA yield ± standard error. (D) Control protocol extracted gDNA is stable through multiple freeze–thaw cycles. Each freeze–thaw eluant was collected separately and is represented as the mean DNA yield normalized to one freeze–thaw (control) ± standard error.