Skip to main content
. Author manuscript; available in PMC: 2020 Jul 4.
Published in final edited form as: Cell Rep. 2020 Jun 9;31(10):107751. doi: 10.1016/j.celrep.2020.107751

Figure 3. Distinct Methylated Forms of H3K36 Are Deposited within or near Transcribed Regions of Genes.

Figure 3.

(A) Schematic of STE11 with amplicons indicated below. (B–D) ChIP analysis of H3K36me3, H3K36me2, and H3K36me1 across STE11 in the indicated strains. The legend in (B) is representative for all the data presented in the figure. (E) Schematic of PMA1 with amplicons indicated below. (F–H) ChIP analysis of H3K36me3, H3K36me2, and H3K36me1 across PMA1 in the indicated strains. (I) Schematic of TDH3 with amplicons indicated below. (J–L) ChIP analysis of H3K36me3, H3K36me2, and H3K36me1 across TDH3 in the indicated strains. Data represented as mean ± SEM of three independent biological replicates. Student’s t test was used to obtain p values. Asterisks indicate significance (*p < 0.05; **p < 0.01); non-significant comparisons not shown. All qPCR primers are listed in Table S3.