KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-Set2 | In House | N/A |
| Anti-G6PDH antibody, polyclonal | Sigma | Cat#A9521–1VL |
| Anti-H3K36me1 | Abcam | ab9048 |
| Anti-H3K36me2 | In House | N/A |
| Anti-H3K36me2 | Active Motif | Cata#39255 |
| Anti-H3K36me2 | Abcam | ab9049 |
| Anti-Histone H3K36me3 | Abcam | ab9050 |
| Anti-H3K27ac | Active Motif | Cat#39133 |
| Anti-H3K56ac | Active Motif | Cat#39281 |
| Anti-histone H3 antibody, polyclonal | EpiCypher | 13–0001 |
| Normal Rabbit IgG | Cell Signaling Technology | 2729 |
| Anti-RNAPII CTD phospho Ser2, clone 3E10 | Active Motif | Cat#61083 |
| Anti-HA Tag | 3ethyl | Cat#A190–108A |
| HRP-conjugated anti-rabbit | GE Healthcare | Cat#NA934V |
| HRP-conjugated anti-mouse | GE Healthcare | Cat#NA931V |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Caffeine | Sigma-Aldrich | Cat#C0750–100G |
| Rapamycin | SIGMA (Roche) | Cat#R8781 |
| Phleomycin | Sigma-Aldrich | Cat#P9546–5MG |
| 6-Azauracil | SIGMA (Roche) | Cat#A1757 |
| 5-FOA | RPI | F10501–10.0 |
| Acid Phenol Chloroform | Ambion/Thermo Fisher Scientific | Cat#AM9722 |
| iTaq Universal SYBR ® Green Supermix | 3iorad | Cat#172–5124 |
| Protein A Agarose | SIGMA (Roche) | Cat #11134515001 |
| FLAG_M2 Agarose beads | Sigma-Aldrich | A2220 |
| Dynabeads Protein G | Thermo Fisher Scientific | Cat#10009D |
| SPRI Beads (Agencourt AMPure XP) | Beckman Coulter | Cat#A63881 |
| ECL Prime | GE Healthcare | Cat#45002401 |
| DNase | Promega | M610A |
| SAM | Sigma-Aldrich | Cat#A7007–25MG |
| Set2 | This Study | N/A |
| set2-Y149F | This study | N/A |
| set2-F234Y | This study | N/A |
| Xenopus nucleosomes | This study | N/A |
| Critical Commercial Assays | ||
| TruSeq Stranded Total RNA with RiboZero Gold Library Prep Kit | Illumina | Cat#RS-122–2301 |
| RNeasy Mini Kit | QIAGEN | Cat#74106 |
| QuikChange II Site-Directed Mutagenesis Kit | Agilent | 200523 |
| ChIP DNA Clean and Concentrator | Zymo | Cat#D5205 |
| Deposited Data | ||
| RNA-seq Data | This study | GEO: GSE125684 |
| Raw data for western blots and spotting assays | This study | Mendeley Data: https://doi.org/10.17632/25fpx9dzsk.1 (https://data.mendeley.com/datasets/25fpx9dzsk/1) |
| Experimental Models: S. cerevisiae Strains | ||
| Yeast Strains | This study and other sources | Table S2 |
| Oligonucleotides | ||
| Oligos | This study and other sources | Table S3 |
| Recombinant DNA | ||
| Plasmids | This study and other sources | Table S4 |
| Software and Algorithms | ||
| ImageJ | Schneider et al., 2012 | https://imagej.nih.gov/ij/ |
| I-TASSER | Zhang, 2008 | https://zhanglab.ccmb.med.umich.edu/I-TASSER/ |
| PyMOL | N/A | https://pymol.org/2/ |
| MODELLER | Webb and Sali, 2016 | https://salilab.org/modeller/ |
| STAR | Dobin et al., 2013 | N/A |
| BEDtools | Quinlan and Hall, 2010 | https://bedtools.readthedocs.io/en/latest/ |
| HOMER | Heinz et al., 2010 | http://homer.ucsd.edu/homer/motif/ |