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. Author manuscript; available in PMC: 2020 Dec 1.
Published in final edited form as: J Biol Inorg Chem. 2019 Nov 14;24(8):1261–1268. doi: 10.1007/s00775-019-01738-2

Fig 4.

Fig 4.

A) Fluorescence spectra of PICUP samples corresponding to NAcαS-CuI (yellow), NAcαS-FeII (green), and NAcαS-FeII followed by immediate addition of CuI (purple). B) SDS-PAGE gel (left) and corresponding anti-dityrosine western blot (right) of PICUP samples of NAcαS and metal combinations involving CuI and/or FeII. C) Fluorescence spectra of PICUP samples corresponding metal bound NAcαS; control NAcαS in the absence of metals (black), NAcαS-CuII (blue), NAcαS-FeII (green), and NAcαS-FeII followed by immediate addition of CuII (orange). D) SDS-PAGE gel (left) and corresponding anti-dityrosine western blot (right) of PICUP samples of NAcαS and metal combinations involving CuII and/or FeII.