Figure 10.

Subunit-dependent potentiation for Cys substitution at the +10 position. (A–C) Whole-cell currents evoked by 10 µM NMDA + 10 µM glycine (open bars) alone or together with coapplication of 50 µM, 500 µM, or 5 mM Cd (cyan bars, left to right) in HEK293 cells transfected with GluN1 WT + GluN2B M10C (A), N1 L10C + N2B WT (B), or N1 L10C + N2B M10C (C). Holding potential is −80 mV. (D and E) Current recorded during exposure to Cd alone (D) or Cd together with agonists (E) plotted as a fraction of current evoked by agonist alone (mean ± SEM, 4–21 cells per condition). Asterisks denote significant difference from WT (one-way ANOVA with post hoc Dunn’s test). (D) Inset shows Application of 50 µM, 500 µM, and 5 mM Cd alone (red bars) caused little change in holding current in a cell transfected with N1L10C + N2B WT.