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. 2020 Apr 20;152(7):e201912524. doi: 10.1085/jgp.201912524

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© 2020 Lamothe and Kurata

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Figure 2. — Slc7a5 promotes an interaction between Kv1.2 and Kvβ. Combinations (as indicated in Fig. 1 A) of Kv1.2, Slc7a5, Kvβ, and GFP were expressed in HEK293 cells. Co-IP showing that the interaction between Kv1.2 and Kvβ is enhanced in the presence of Slc7a5. GAPDH was used as a negative control. IgG is used as the loading control. WB, Western blotting; IB, immunoblotting. Consistent results were obtained from four independent co-IP experiments (Fig. S2).