Figure S6.

A high Kvβ:Kv1.2 transfection ratio does not cause accelerated inactivation and does not prevent functional effects of Slc7a5. (A) LM cells were transfected with Kv1.2(T252R) and Kvβ in the absence or presence of mCherry Slc7a5. Transfection ratios were (with Kv1.2 maintained constant using GFP) Kv1.2:Kvβ, 1:10; Kv1.2:Kvβ:Slc7a5, 1:10:0.5. Cells were hyperpolarized to −120 mV for 30 s before recording to disinhibit Kv1.2 currents. Time constants of inactivation (τ inact) were calculated from a single exponential fit of the inactivating current at depolarizing sweeps from 50 to 110 mV in 10-mV increments for 100 ms from a −100-mV holding potential (mean ± SEM; n = 7–12 individual cells recorded for each group). (B) τ Inactivation of individual cells from each group in A were obtained from the inactivating currents at +100 mV from a −100-mV holding voltage (n = 7–12 individual cells recorded for each group). Student’s t test was used to compare the Slc7a5 treatment group versus the respective control; P values are denoted above the box plots. Box plots depict the median, 25th, and 75th percentiles (box) and the 10th and 90th percentiles (whiskers).