Figure 6.

Axonal development of SAD-A-deficient hippocampal neurons. (A) Representative images of hippocampal neurons cultured from Sada heterozygous (+/∆ex2, left) and homozygous (∆ex2/∆ex2, right) mouse brains. Hippocampal neurons were cultured for 7 days and stained with anti-pJNK (axonal marker, green), anti-MAP2 (dendrite marker, red), and DAPI (blue). Bar, 100 μm. (B) Quantification of neurite length. The ratio of the longest pJNK-positive/MAP2-positive neurite length (left), the total number of MAP2-positive neurites (middle), and the total length of pJNK-positive neurites (right). Closed bar, +/∆ex2, n = 58 neurons; open bar, ∆ex2/∆ex2, n = 35 neurons. ***P < 0.001.