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. 2020 Jul 3;10:11010. doi: 10.1038/s41598-020-66981-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The proportion of Treg (CD4⁺CD25⁺CD127 ^dim/-) cells in CD4⁺T cells from the Buffy Coat of healthy donors. CD25 and CD127 expression were determined by flow cytometry analysis in CD4⁺T cells after 6 days culture with Treg-polarizing cytokines, with PMA and Ionomycin stimulation. Anti-PC flow, Anti-PC IgM, Anti-MDA flow and Anti-MDA IgM were added one day before harvest. The percentage ±SD of three different cultures performed. Differences between Treg cells Anti-MDA IgM treatment showed statistically significant. (p < 0.05). Differences between Treg cells Anti-MDA IgM treatment showed no statistically significant effects. Effects of anti-PC and anti-MDA on Treg polarization.