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. 2020 Jul 3;10:11010. doi: 10.1038/s41598-020-66981-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Proteomics analysis. Similarities and differences between the polyclonal anti-PC and anti-MDA IgM variable regions. Differences in the heavy variable chain CDR2 and CDR3 regions between polyclonal anti-PC IgM and non-anti-PC IgM (flowthrough, FT) as well as anti-MDA and anti-MDA-FT samples. The data was generated via quantitative proteomics de novo sequencing analysis. Numbers are reported as log(ppm) of the relative variable region peptide distributions in respective sample. (A) Peptides from the HV CDR3 region that were elevated in the anti-MDA IgM and/or anti-PC samples. (B) Peptides from the HV CDR2 region that were elevated in the anti-PC IgM samples.