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. 2020 Jul 3;11(7):500. doi: 10.1038/s41419-020-2711-x

Fig. 2. MiR-1976 knockdown promoted migration and invasion in vitro.

Fig. 2

a The expression levels of miR-1976 in breast cancer cells transfected with miR-1976 mimics and inhibitor lentivirus respectively were tested by qRT-PCR. b–d The wound healing assays were performed to assess the effect of miR-1976 on cell motility at 0 and 48 h. Scale bar: 100 μm. e, f The transwell migration and invasion assays were performed to detect the effects of miR-1976 on migration and invasion. Scale bar: 100 μm. g Western blot analysis showed that miR-1976 mimics increased the level of E-Cadherin, and reduced the levels of N-Cadherin, Vimentin, Snail, and Slug in SUM-1315 and MDA-MB-231, vice versa in ZR-75-1 and MCF-7 transfected with miR-1976 inhibitor. *p < 0.05, **p < 0.01, ***p < 0.001. The data expressed as the mean ± SD.