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. 2020 Jul 3;11(7):500. doi: 10.1038/s41419-020-2711-x

Fig. 3. MiR-1976 knockdown changed CSC properties.

Fig. 3

a The expression levels of CD44 and CD24 in cells transfected with miR-1976 mimics and inhibitor respectively were analyzed by flow cytometry analysis. Cells were stained with anti-CD44-APC and anti-CD24-PE. Overexpression of miR-1976 decreased the populations of CD44+/CD24− in SUM-1315 and MDA-MB-231, and miR-1976 knockdown increased the populations of CD44+/CD24− in ZR-75-1 and MCF-7. b Mammosphere formation assay was performed, and the representative pictures were shown. Scale bar: 100 μm. c Western blot analysis showed that miR-1976 reduced the protein expression level of CD44 in SUM-1315 and MDA-MB-231, and miR-1976 inhibitors increased the level in ZR-75-1 and MCF-7. *p < 0.05, **p < 0.01, ***p < 0.001. The data expressed as the mean ± SD.