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. 2020 Jul 3;11(7):500. doi: 10.1038/s41419-020-2711-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — SUM-1315 transfected with miR-1976 mimics and negative control were injected into the tail vein of female nude mice. a Table listed the incidence of metastasis in the nude mice treated with SUM-1315 transfected with miR-1976 mimics and negative control. b IHC analyses of E-Cadherin, N-Cadherin, and Vimentin in lung metastases. The positive staining was indicated by a brown color. The expression level of E-Cadherin in the control group was lower than that in the other group injected with miR-1976 mimics, while the expression levels of N-Cadherin and Vimentin in the control group were markedly higher. Scale bars: 100 μm. c IHC analyses of CD44 in lung metastases. The positive staining was indicated by a brown color. The expression level of CD44 in the control group was significantly higher than that in the other group injected with miR-1976 mimics. Scale bars: 100 μm.